Mitochondrial Ceramide-Rich Macrodomains Functionalize Bax upon Irradiation

Mitochondrial Ceramide-Rich Macrodomains Functionalize Bax upon Irradiation

BACKGROUND Evidence indicates that Bax functions as a "lipidic" pore to regulate mitochondrial outer membrane permeabilization (MOMP), the apoptosis commitment step, through unknown membrane elements. Here we show mitochondrial ceramide elevation facilitates MOMP-mediated cytochrome c release in HeLa cells by generating a previously-unrecognized mitochondrial ceramide-rich macrodomain (MCRM), w...

Ceramide Permeabilization of Mitochondrial Outer Membrane: Pharmacological Characterization and Relation to Mac and Bax

Title of Document: CERAMIDE PERMEABILIZATION OF MITOCHONDRIAL OUTER MEMBRANE: PHARMACOLOGICAL CHARACTERIZATION AND RELATION TO MAC AND BAX Suzanne J. Hudak, Master of Science, 2005 Directed By: Dr. Marco Colombini, Professor, Biology and Biochemistry Departments In apoptosis, the mitochondrial outer membrane (MOM) becomes permeable, releasing proteins. This permeability has been attributed to t...

Mitochondrial Ceramide in Stroke

HSC senescence upon irradiation.

In this issue of Blood, Shao et al report that a side effect of total body irradiation (TBI) is long-term bone marrow injury and thus dysfunctional hematopoiesis caused by the induction of hematopoietic stem cell (HSC) senescence. Interestingly and unexpectedly, however, this happens in a manner independent of the cell-cycle regulators Ink4a and Arf, which play a major role in senescence in oth...

Ceramide-rich membrane rafts mediate CD40 clustering.

Many receptor systems use receptor clustering for transmembrane signaling. In this study, we show that acid sphingomyelinase (ASM) is essential for the clustering of CD40. Stimulation of lymphocytes via CD40 ligation results in ASM translocation from intracellular stores, most likely vesicles, into distinct membrane domains on the extracellular surface of the plasma membrane. Surface ASM initia...